Core Rsch Facil Technician III - NextGen DNA Sequencing

• Performs QC of samples submitted to the NGS lab. This includes assessing the quantity and quality of materials to determine suitability for NextGen DNA sequencing procedures.
• Submits samples for qPCR to the Gene Expression core
• Constructs Illumina libraries for sequencing on Illumina platforms
• Properly tracks samples and documents laboratory work
• Communicates with supervisor and other core personnel about experimental results in a timely and professional manner
• Works with the supervisor to define deliverables and timelines for project completion
• Tests, troubleshoots, and implements new protocols for library construction, DNA shearing, sample cleanup, and size selection, using novel procedures as they become available in the literature or from vendors
• Contributes to the preparation, updating, and organization of Standard Operating Procedures (SOPs)

General Lab Operation and Maintenance Functions

• Performs maintenance tasks associated with the upkeep of the NGS lab, such as the proper disposal of biohazard trash, receiving and storing of samples and reagents
• Monitors reagents and consumables and regularly updates inventories
• Assists with the administrative duties necessary for proper handling of receipts and orders
• Revision of sample sheets, tracking, and disposal of samples in MyBiotech
• Freeze and fridge maintenance and documentation in MyBiotech
• Other duties as assigned

Do you have experience in Windows?, Do you have a Bachelor’s degree?, BA/BS in a related scientific field, or an equivalent combination of education and experience. Core or technology-specific licensure and/or certifications, where applicable. Additionally, yearly certificate and compliance training, if required. PREFERRED:

The preferred applicant will have a BS/BA in Molecular Biology or a related scientific field with familiarity of in basic techniques and instrumentation used in the molecular biology laboratory, including specific NGS procedures that include (but are not limited to):

• Genomic DNA purification, quantification, and manipulation; PCR; NGS DNA library construction and quality assessment/evaluation.
• Use of robotics for high-throughput processing of nucleic acids and DNA library samples.
• Hands-on experience with Illumina, PacBio, and/or ONT NGS instrumentation and technology.
• Basic laboratory citizenship, including organization of equipment and supply inventory.
• Instrument run coordination and maintenance.
• Preparation of common buffers and solutions.
• Computer literacy in Windows/UNIX systems, Microsoft Office package.
• Familiarity with utilizing Standard Operating Procedures (SOPs), iLAB, and laboratory information management system (LIMS).