Job offer

Participation in the development of three-dimensional cortical models with improved spatial organization and stratification.

Quantitative analysis of imaging data and collaboration with PatCorg consortium partners.The candidate should hold a master’s degree or equivalent in cell and developmental biology, bioengineering, biophysics, neuroscience, biomedical engineering, or microfluidics. Experience in at least one of the following areas would be particularly appreciated:

1. pluripotent stem cells or organoids;

2. development of the nervous system;

3. microfluidics or organs-on-a-chip;

4. microscopy and quantitative image analysis;

5. Scientific programming, including Python, Fiji/ImageJ, or MATLAB.

A strong motivation for interdisciplinary projects at the interface between developmental biology, neuroscience and bioengineering will be essential., PhD or equivalent

Research Field Biological sciences, The candidate should hold a master’s degree or equivalent in cell and developmental biology, bioengineering, biophysics, neuroscience, biomedical engineering, or microfluidics. Experience in at least one of the following areas would be particularly appreciated:

1. pluripotent stem cells or organoids;

2. development of the nervous system;

3. microfluidics or organs-on-a-chip;

4. microscopy and quantitative image analysis;

5. Scientific programming, including Python, Fiji/ImageJ, or MATLAB.

A strong motivation for interdisciplinary projects at the interface between developmental biology, neuroscience and bioengineering will be essential.

We offer a highly interdisciplinary, inclusive and international work environment at the Cell and Cancer Physics Unit (CNRS, Institut Curie, PSL, SU). Located in the heart of the rich Latin Quarter in Paris, our unit hosts experimental and theoretical physicists, biologists and biochemists. We are deeply committed to fostering diversity and are looking for talented scientists in all fields at the physics-biology interface.

Brain organoids are major models of human brain development, but they still imperfectly reproduce the regionalisation and layering of the cortex. The ERC Synergy PatCorg project aims to create organoids that more closely recapitulate the organisation of the human cortex, using microfluidics.

The objective of the doctoral project will be to develop a cortex-on-chip model to spatially and temporally control the delivery of morphogens in order to induce cortical organizing centers and to study the formation of regionalization axes comparable to the anterior-posterior and medio-lateral axes of the developing cortex. Activities, Main missions Culture and differentiation of human pluripotent stem cells into neuroepithelium and cortical organoids. Use and optimization of μ-transwell microfluidic devices to locally deliver morphogens on the basal surface of the tissue. Study of the influence of concentration, duration of exposure, combinations of morphogens and distance between organizing centers. Analysis of cortical regionalization by imaging and quantification of transcription factor gradients, including EMX2, PAX6 and SP8.